賈定洪; 鄭林用; 王波; 郭勇; 甘炳成; 黃忠乾; 彭衛紅
四川省農業科學院土壤肥料研究所
【中文摘要】 以7個栽培菌株為參照,采用ISSR分子標記技術對5個野生木耳屬菌株進行分析,使用NTSYSpc2.1生物軟件對12個供試菌株進行聚類分析,構建系統樹。試驗篩選出了11個擴增譜帶清晰、多態性好的ISSR引物,共擴增出78條清晰易辨的多態性譜帶。聚類分析結果表明,利用ISSR技術可將全部供試材料區分開,遺傳相似系數變異范圍為0.1080.822,在相似系數為0.51時,12個菌株聚為6個群,其中5個野生菌株各自聚為不同類群,遺傳差異較大。
【英文摘要】 Five wild-type Auricularia strains were identified by ISSR(inter-simple sequence repeat)methodology using seven cultivated Auricularia strains as controls.Eleven ISSR primers,selected from a total of 60 test ISSR primers,amplified 78 distinct polymorphic DNA bands from the 12 Auricularia strains.ISSR similarity coefficients ranged from 0.108 to 0.822,and a dendrogram based on UPGMA(unweighted pair group method with arithmetic average) cluster analysis using these values revealed that the 12 strains were clustered into six groups(similarity coefficient 0.51).The five wild-type strains clustered into five different groups,and the most distinct strain genetically was the wild-type strain,Ausm(similarity coefficient 0.198).
【中文關鍵詞】 木耳屬; ISSR; 遺傳鑒定
【英文關鍵詞】 Auricularia; inter-simple sequence repeats; genetic identification
【基金】四川省財政育種工程資助項目“食用菌新品種選育及新材料新方法研究”(編號:2006YZCC09-024);; 四川省育種攻關資助項目“優質豐產黑木耳新品種選育”(編號:YZGG06-04-01)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年03期 【DOI】CNKI:SUN:SYJB.0.2008-03-002