黃龍花; 吳清平; 楊小兵; 張菊梅
中國科學院南海海洋研究所; 廣東省微生物研究所; 廣東省菌種保藏與應用重點實驗室; 中國科學院研究生院
【中文摘要】 針對我國栽培鳳尾菇(Pleurotus sajor-caju)及其近緣種共10個菌株的ITS序列,應用特異引物進行PCR擴增,對其產物進行測序,并從GenBank上下載側耳屬(Pleurotus)3個種7條ITS序列、韌傘屬(Lentinus)的4條ITS序列和香菇屬(Lentinula)的3條ITS序列,以冬蟲夏草(Cordyceps sinensis)和蛹蟲草(Cordyceps militaris)為外類群,用NJ(Neighbor-joining)法構建系統發育樹,結果表明:鳳尾菇在側耳屬分支上與側耳屬內種的相似性達98.5%以上,韌傘屬和香菇屬的屬內相似性也均為98%以上,而側耳屬和韌傘屬、香菇屬的屬間相似性僅約80%,故鳳尾菇應歸于側耳屬;在進一步研究中,將該鳳尾菇與其近緣種的ITS1-5.8S-ITS2序列作堿基完全比對,結果發現它與肺形側耳(Pleurotus pulmonarius)的堿基完全一致,而與其它近緣種均存在較大堿基差異,因此,目前我國廣泛栽培的鳳尾菇與肺形側耳可能是同物異名種,建議訂正其名稱,將其拉丁名統一為P.pulmonarius。本研究首次將ITS序...更多列分析和堿基比對結合對鳳尾菇的分類地位及拉丁名進行研究,結果表明ITS序列分析在近緣種的分類鑒定上很有價值。 還原
【英文摘要】 In order to clarify the taxonomic status of Pleurotus sajor-caju,we determined ITS sequences from six Pleurotus (including one strain of P. sajor-caju) and four Lentinula strains cultivated in China. Data were combined with ITS sequences from 11 related strains,including Pleurotus,Lentinus,Lentinula,Cordyceps and Ophiocordyceps,obtained from GenBank to construct a phylogenetic tree using the Neighbor-joining method with Cordyceps militaris and Ophiocordyceps sinensis as the outgroups. The P. sajor-caju test strain was located on a branch that incorporated all the other Pleurotus strains examined with a shared similarity value >98.5%. Similarity values among Lentinus and Lentinula strains was also >98%,but only 80% when all three genera were compared. In a further "complete alignment"study,comparison of sections of ITS1-5.8S-ITS2 sequences from four P. sajor-caju strains with corresponding sequences from strains of P. pulmonarius,P.ostreatus and P. eryngii revealed that the sequen...更多ces from P. sajor-caju and P. pulmonarius strains were identical but were different from the other Pleurotus strains. Our data indicate that the P. sajor-caju test strain was synonymous with P. pulmonarius and recommend that it be renamed accordingly. 還原
【中文關鍵詞】 鳳尾菇; 肺形側耳; ITS序列; 系統發育樹
【英文關鍵詞】 Pleurotus sajor-caju; Pleurotus pulmonarius; ITS sequence; phylogenetic tree
【基金】廣東省科技計劃項目(編號:2008B050300001,2008A020100024)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-006