趙風云; 林俊芳; 葉澤波; 王藝紅; 楊博; 郭麗瓊
華南農業大學食品學院生物工程系; 華南農業大學生物質能研究所
【中文摘要】 草菇V23原生質體再生菌絲經過0℃低溫脅迫處理108 h后,篩選出菌絲較耐低溫的菌株V23a,獲得V23a的孢子分離菌株(Spore Isolation Strains,SIS),經栽培后,篩選生物學效率高的菌株進行組織分離,獲得組織分離菌株(Tissue Isolation Strains,TIS),選出其中生物學效率最高的Va2作為草菇高產新菌種,其生物學效率比對照V23高14.27%。選育過程中分析了SIS菌株的氣生菌絲密度、厚垣孢子數量和出菇率,比較了SIS和TIS菌株的菌絲生長速度、原基數、生產周期、單菇平均質量和生物學效率等主要性狀。結果表明,可出菇的SIS菌株其子實體產量差異較大,但均低于對照菌株V23。TIS菌株的產量比SIS的提高了087.18%。
【英文摘要】 Volvariella volvacea,strain V23a,was selected from regenerated protoplasts of strain V.volvacea V23 by exposure to 0 ℃ for 108 h.Spore Isolated Strains(SIS) were obtained in relatively high yield from V23a fruit bodies using standard methodology,and Tissue Isolated Strains(TIS) were then isolated from fruit bodies of the SIS.A new high-yielding(14.27% higher yield compared with strain V23) V.volvacea strain,Va2,was selected from the TIS.Aerial hyphae and chlamydospore production,and fruiting frequency values,were determined for SIS,and mycelial growth rates,densities of primordia,times elapsing between substrate inoculation and harvesting of the fruit bodies,average fruit body weights and Biological Efficiency values were recorded for both SIS and TIS.Fruit body yields for SIS varied considerably and were invariably lower compared to strain V23,whereas yields for TIS were up to 87.2 % higher than those of the corresponding SIS.
【中文關鍵詞】 草菇; 孢子分離; 組織分離; 生物學效率
【英文關鍵詞】 Volvariella volvacea; spore isolation; tissue isolation; Biological Efficiency
【基金】國家高技術研究發展計劃“利用食用菌工程菌株創制食品專用的多功能纖維素酶”(863計劃,編號:2006AA10Z301);; 廣東省科技計劃課題“食用菌產業推進關鍵技術研究與示范”(編號:2008A02010024)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-005