江玉姬; 王秀全; 劉維俠; 謝寶貴; 朱堅
福建農林大學菌物研究中心; 福建農林大學食品科學學院; 中國熱帶農業科學院橡膠研究所; 福建農林大學園藝學院
【中文摘要】 從29株金針菇(Flammulina velutipes)中經單孢分離獲得的單核菌株,兩兩配對后,在顯微鏡下觀察鎖狀聯合,配對結果將金針菇的A因子確定到10個,B因子確定到8個。13個黃色菌株的不親和性因子包含了從A1到A9,B1到B8;而16個白色菌株的不親和性因子大多為A2A3B2B3類型。有24個金針菇菌株獲得的單核菌絲有4種交配型且符合1∶1∶1∶1比例,其它菌株獲得的單核菌絲交配型與理論數據有偏離。
【英文摘要】 Monokaryons isolated from single spores of 29 Flammulina velutipes strains were mated with each other and the formation of dikaryons determined by the presence of clamp connections.Ten A and eight B mating factors were identified.Mating types of 13 yellow strains consisted of different combinations of A1 to A9 and B1 to B8 mating factors,whereas the majority of white strains were of the A2A3B2B3 mating type.The four mating types observed among the monokaryons derived from 24 strains were present in a 1∶1∶1∶1 ratio whereas mating types in monokaryons derived from the remaining strains deviated from the theoretical distribution.
【中文關鍵詞】 金針菇; 不親和性因子; 遺傳關系; 交配型
【英文關鍵詞】 Flammulina velutipes; incompatibility factors; genetic relationship; mating type
【基金】福建省自然科學基金資助項目“金針菇遺傳連鎖群構建的研究”(編號:2006J055);; 福建省科技廳資助項目“金針菇工廠化栽培專用品種的選育與示范”(編號:2007N0003);; 福州市科技局計劃項目“金針菇工廠周年生產智能化控制技術的研究與示范”[編號:榕科(2007)109號]的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-003