【作者】陳自宏; 虞泓; 曾文波; 楊俊媛; 袁靜; 陳毅堅
【Author】CHEN Zihong; YU Hong; ZENG Wenbo; YANG Junyuan; YUAN Jing; CHEN Yijian Yunnan Herbal Laboratory; Institute of Herb Biotic Resources; Yunnan University; Kunming; Yunnan 650091; China
【作者機構】云南大學中草藥生物資源研究所云百草實驗室
【摘要】 以蟲草素含量為指標,采用正交實驗優化了適合擬黑蟲草(Cordyceps nigrella)菌株cnig-56固體發酵的培養基,并探討了該菌株固體發酵的最佳條件。結果表明,菌齡為3d的擬黑蟲草菌種按10%接種量接入優化的固體培養基上(配方為大米粉9.9%,青稞粉6.6%,玉米粉9.9%,蠶蛹粉3.3%,粗麩皮70.15%,KH2PO40.05%,MgSO40.1%)于24℃發酵培養30d,其產物的蟲草素含量最高為101μg/g。
【Abstract】 Orthogonal testing was used to optimize selected parameters affecting cordycepin production by Cordyceps nigrella (strain cnig-56) grown under solid-state conditions. Highest cordycepin levels (101 μg/g) were recorded after 30 d cultivation at 24 ℃ on a medium consisting of 9.9% rice powder,6.6% highland barley powder,9.9% corn powder,3.3% dried silkworm chrysalis powder,70.15% unrefined wheat bran,0.05% KH2PO4,0.1% MgSO4·7H2O,a solid:water ratio of 1:1.2,and using 3-day-old mycelium (10% w/w) as inoculum.
【關鍵詞】 擬黑蟲草; 固體培養; 正交實驗; 蟲草素
【Key words】 Cordyceps nigrella; solid fermentation; orthogonal test; cordycepin
【基金】云南省自然科學基金重點項目(編號:2008CC019)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年01期
【DOI】CNKI:SUN:SYJB.0.2010-01-019
【分類號】S567.35