大球蓋菇原生質體再生及單核化特性的研究
閆培生 李桂舫 蔣家惠 黃健
摘 要：大球蓋菇原生質體再生條件及單核化特性結果。原生質體再生速度極快，涂布平板3d后肉眼即可見明顯的再生菌落形成,在PGPM再生培養基上再生率為0.97～2.0%，滲穩劑種類對再生率無明顯影響，但可影響再生菌落形態,液體預培養1～2d，再生率明顯下降；大球蓋菇原生質體單核化率高達77.6%，且再生雙核體和再生單核體在形成再生菌落時無時間差，其生長速度亦無快慢之分，液體預培養可顯著減少單核化率，再生單核體中存在親本兩種交配型，但二者的比率不為1。
關鍵詞：大球蓋菇，原生質體，單核化
分類號：Q932 文獻標識碼：A
文章編號：1007-3515（2001）01-0107-0110
REGENERATION AND MONOKARYOTATION OF PROTOPLASTS OF STROPHARIA RUGOSO-ANNULAT
YAN Pei-Sheng（Institute of Applied Mycology, Department of Plant Protection, Laiyang Agricultural University, Laiyang, Shandong 265200）
LI Gui-Fang（Institute of Applied Mycology, Department of Plant Protection, Laiyang Agricultural University, Laiyang, Shandong 265200）
JIANG Jia-Hui（Institute of Applied Mycology, Department of Plant Protection, Laiyang Agricultural University, Laiyang, Shandong 265200）
HUANG Jian（Department of Biology, Qingdao Educational College, Qingdao 266071）
Abstract：Regeneration and monokaryotation of Stropharia rugoso-annulata protoplasts were investigated. The results showed that the protoplast regeneration was very fast, and the regenerated colonies could be seen by naked eyes after plating 3d. The regeneration rate was 0.97～2.00% on PGPM. The osmotic stabilizers of mannitol, sucrose and magnesium sulphate had no significant effects on regeneration rate, but could affect the morphology of regenerated colonies. Pre-culture of protoplasts in liquid regeneration media for 1～2d at 25℃ could decrease the regeneration and monokaryotation rate. The protoplast monokaryotation rate was 77.7%. The regenerated dikaryons and monokaryons could be formed on the plates simultaneously, and had no difference in mycelium growth rate and colony-forming time. Two kinds of parent mating types could be found among protoplasted monokaryons, but the ratio was not 1:1. It was the first report about the regeneration and monokaryotation characters of Strapharia rugoso-annulata.
Keywords：Stropharia rugoso-annulata, Protoplast, Monokaryotation
基金項目：山東省教委資助課題
作者簡介：閆培生,E-mail: psyan@lyac.edu.cn
作者單位：閆培生（萊陽農學院植保系應用真菌研究室 山東 萊陽265200； 2青島教育學院 青島 266071）
李桂舫（萊陽農學院植保系應用真菌研究室 山東 萊陽265200；）
蔣家惠（萊陽農學院植保系應用真菌研究室 山東 萊陽265200；）
黃?。ㄇ鄭u教育學院 青島 266071）
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