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          中國栽培白靈側耳的RAPD和IGS分析


          【發布日期】:2005-01-06  【來源】:
          【核心提示】:中國栽培白靈側耳的RAPD和IGS分析RAPDANDIGSANALYSISOFPLEUROTUSNEBRODENSISCULTIVARSINCHINA張金霞 黃晨陽 張瑞穎

          中國栽培白靈側耳的RAPD和IGS分析

          RAPD AND IGS ANALYSIS OF PLEUROTUS NEBRODENSIS CULTIVARS IN CHINA

          張金霞 黃晨陽 張瑞穎 管桂萍 

          摘 要:以RAPD和IGS為分子標記,研究了中國栽培白靈側耳Pleurotus nebrodensis菌株的遺傳多樣性,RAPD結果表明,供試菌株間存在多態性;對供試菌株的IGS分析表明,白靈側耳P.nebrodensis的IGSl區域較保守,菌株間沒有多態性;IGS2區域進化較快,菌株間具豐富的多態性.IGS2-RFLP可應用于白靈側耳的菌株鑒定鑒別,較RAPD更穩定,更具可操作性.

          關鍵詞:遺傳多樣性,菌株多態性,IGS2-RFLP

          分類號:Q939.5  文獻標識碼:A

          文章編號:1672-6472(2004)04-0514-0519

          基金項目:引進國際先進農業科學技術("948"計劃)項目(2004-Z21)

          作者單位:張金霞(中國農業科學院土壤肥料研究所,北京,100081)黃晨陽(中國農業科學院土壤肥料研究所,北京,100081)張瑞穎(中國農業科學院土壤肥料研究所,北京,100081)管桂萍(中國農業科學院土壤肥料研究所,北京,100081) 

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          收稿日期:2004年5月24日

          修稿日期:2004年7月1日

          出版日期:2004年11月22日

          菌物學報

          MYCOSYSTEMA

          2004 Vol.23 No.4 P.514-519

           
           
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