臺灣蟲草的被毛孢無性型新種及其對黃粉蟲的侵染研究 李春如 夏成潤 林英任 樊美珍 李增智 摘 要：報道采自安徽省黃山風景區的臺灣蟲草Cordycepsformosana,多批次收集彈射的子囊孢子分離得到其無性型,經鑒定為一被毛孢新種,命名為黃山被毛孢Hirsutella huangshanensis.黃山被毛孢在米飯培養和自制的蟲草培養基上均能長出子實體,其菌落及子實體顏色與天然蟲草較為相似,但未見成熟的子囊殼.應用黃山被毛孢RCEF0868菌株對飼喂昆蟲黃粉蟲進行感染試驗,結果表明:黃粉蟲在菌落接觸感染、剛蛻皮幼蟲的穿刺接種和孢子濃度1.0×107/ml浸蘸處理下,獲得了相對較高的侵染率,分別達到30%、25%和25%,剛蛻皮幼蟲一般比深色幼蟲組其侵染率較高,1×105/ml孢子濃度浸蘸試蟲不論是剛蛻皮或老熟幼蟲均不能感染.10d后被感染的黃粉蟲形成僵蟲,繼續保濕培養20d后,體表節間膜處形成白色菌膜,粘片鏡檢表明感染真菌即為供試菌株.40d后,可見子實體原基及孢梗束,尚未見成熟的子囊殼. 關鍵詞：黃山被毛孢;蟲生真菌;侵染率 分類號：Q939.5 文獻標識碼：A 文章編號：1672-6472(2005)03-0349-0355 HIRSUTELLA HUANGSHANENSIS SP.NOV., THE ANAMORPH OF CORDYCEPS FORMOSANA AND ITS INFECTION ON TENEBRIO MOLITOR LI Chun-Ru XIA Cheng-Run LIN Ying-Ren FAN Mei-Zhen LI Zeng-Zhi 基金項目：安徽省自然科學基金資助 作者簡介：樊美珍,Corresponding author:mzfan@ahau.edu.cn 作者單位：李春如（安徽農業大學微生物防治省重點實驗室,合肥,230036;安徽醫科大學基礎醫學院,合肥,230032） 夏成潤（安徽農業大學微生物防治省重點實驗室,合肥,230036） 林英任（安徽農業大學微生物防治省重點實驗室,合肥,230036） 樊美珍（安徽農業大學微生物防治省重點實驗室,合肥,230036） 李增智（安徽農業大學微生物防治省重點實驗室,合肥,230036） 參考文獻： [1]Hywel-Jones NL, 1997. Hirsutella species associated with hoppers (Homoptera) in Thailand. Mycol Res, 101 (10): 1202～1206 [2]Kobayasi Y, 1941. The genus Cordyceps and its allies. Sci. Rep. Tokyo Bun. Daig. Ser. B. 5, No, 84:53～260 [3]Kobayasi Y, Shimizu D, 1981. The genus Cordyceps and its allies from Taiwan (Formosa). Bull Natn Sci Mus, 7 (4): 113～122 [4]Kobayasi Y, 1982. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans Mycol Soc Jap, 23:329～364 [5]Kobayasi Y, Shimizu D, 1983. Iconography of Vegetable Wasps and Plant Worms. Osaka: Hoikusha Publishing Co., Ltd. 1～280 [6]Li CR, 2003. Research development on anamorph of Cordyceps during the past ten years. Research and Application of Entomogenous Fungi in China, 5:45～53 (in Chinese) [7]Li CR, Fan MZ, Huang B, Wang SB, Li ZZ, 2002. The genus Cordyceps and its allies fromAnhui I. Mycosystema, 21 (2): 167～171 [8]Li, CR, Fan MZ, Huang B, Li ZZ, 2001. Hirsutella longissima sp. nov., the anamorph of Cordyceps longissma. Mycosystema, 20 (1):29～34 (in Chinese) [9]Liang ZQ, 1985. Isolation and identification of conidial stage of Cordyceps gunnii. Acta Mycologica Sinica, 4:162～166 (in Chinese) [10]Liang ZQ, 1990a. Studies on classification of the genus Hirsutella Pat. I. Advances and the characteristics of taxonomy. Journal of GuizhouAgric Coll, 9 (1): 58～68 (in Chinese) [11]Liang ZQ, 1990b. Classification of the genus Hirsutella Pat. Ⅱ. Key of species to the genus Hirsutella Pat. Southwest China Journal of Agricultural Sciences, 3 (3): 32～39 (in Chinese) [12]Liang ZQ, 1991. Two new entomopathogenic fungi of the genus Hirsutella. Southwest China Journal of Agricultural Sciences,4 (1): 51～56 (in Chinese) [13]Sato H, Shimazu M, 2002. Stromata production for Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Appl Entomol Zool, 37 (1): 85～92 [14]Shimizu D, 1994. Color Iconography of Vegetable Wasps and Plant Worms. Tokyo: Seibundo Shinkosha. 1～380 [15]Wang GD, 1995. Cordyceps spp.: Ecology, Cultivation and Application. Beijing: Scientific and Technical Literature Publishing House.1～307 (in Chinese) [16]李春如,2003.近十年來蟲草無性型研究進展.中國蟲生真菌研究與應用(第五卷).北京:中國農業科學技術出版社,45～53 [17]李春如,樊美珍,黃勃,李增智,2001.被毛孢屬一新種--長座蟲草的無性型.菌物系統,20(1):29～34 [18]梁宗琦,1985.古尼蟲草分生孢子階段的分離和鑒定.真菌學報,4(3):162～166 [19]梁宗琦,1990a.被毛孢屬(Hirsutella Pat.)的分類研究Ⅰ.進展及分類特征.貴州農學院學報,9(1):58～68 [20]梁宗琦,1990b.被毛孢屬的分類研究Ⅱ.已知種的檢索.西南農業學報,3(3):32～39 [21]梁宗琦,1991.被毛孢屬的兩種新昆蟲病原真菌.西南農業學報,4(1):51～56 [22]王國棟,1995.冬蟲夏草類生態培植應用.北京:科學技術文獻出版社.1～307 菌物學報 2005年第3期-HTM文件 No.8
