真菌完整染色體DNA的幾種不同制備方法
Several Different Preparation Methods for Intact Chromosome DNA of Fungi
楊建明 馮愛萍 陳明杰 潘迎捷
摘 要：系統地介紹了目前用于真菌電泳核型分析的真菌完整染色體DNA的7種制備方法,并討論了各種方法在真菌中應用的優缺點,為真菌電泳核型分析提供技術基礎.
關鍵詞：電泳核型; 完整染色體DNA; 原生質體法; 液氮研磨法; 直接包埋電泳法
分類號：Q949.32;Q343.2 文獻標識碼：A
文章編號：1005-9873(2003)02-0055-05
基金項目：本項目由上海市科學技術發展基金資助,項目編號(003912076)
作者簡介：楊建明(1978-),男,2001年畢業于安徽技術師范學院生物系,學士學位,現為南京農業大學生命科學學院微生物學系在讀碩士研究生.研究方向: 真菌遺傳育種.陳明杰,本文通訊作者
作者單位：楊建明（南京農業大學生命科學學院,南京,210095）
馮愛萍（農業部食用菌遺傳育種重點開放實驗室;上海市農業遺傳育種重點開放實驗室;上海市農業科學院食用菌研究所,上海,201106）
陳明杰（農業部食用菌遺傳育種重點開放實驗室;上海市農業遺傳育種重點開放實驗室;上海市農業科學院食用菌研究所,上海,201106）
潘迎捷（農業部食用菌遺傳育種重點開放實驗室;上海市農業遺傳育種重點開放實驗室;上海市農業科學院食用菌研究所,上海,201106）
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