施慶利; 羅信昌
華中農業大學應用真菌研究所
【中文摘要】 本研究以ＲＡＰＤ（ＲａｎｄｏｍｌｙＡｍｐｌｉｆｉｅｄＰｏｌｙ－ｍｏｒｐｈｉｃＤＮＡ）技術為主，比較分析食用菌木耳屬（Ａｕｒｉｃｕｌａｒｉａ）種內雜交種．種間原生質體融合子與其親本菌株的ＰＣＲ（ＰｏｌｙｍｅｒｓｅＣｈａｉｈＲｅａｃｔｉｏｎ）擴增的ＤＮＡ片段之間差異，并輔以其它手段，如核相及核數目觀察，同功酶鑒別，以期建立完整有效的檢測技術系列，確證其雜交種融合子的真實性及可靠性。在ＲＡＰＤ分析中，用于測試的１０種隨機引物僅有４種引物與供試菌株的ＤＮＡ模板具有較好的結合能力，其中１種引物可用于檢測雜交種及其親本，另外３種引物可用于檢測融合子反親本。檢測結果顯示：３個供試雜交種中有２個為真雜合子，１個為假雜合子；２個供試融合子均為非穩定雜合子。與常規方法鑒定結果比較基本相符。因此，ＲＡＰＤ技術提供了一種快速、簡便、準確的雜交種及融合子的鑒別方法。
【英文摘要】 RAPD(Randomy Amplified Polymorphic DNA)was used to analysethe differences of DNA fragments between intraspecies hybrid,interspecies protoplastfusants and their respective parents in genus Auricularia,With the help of other methodssuch as the nuclear number in the hyphal cell, the comparison of the isozyme graphs, the hybrids and the protoplast fusants were identified.Four of ten arbitrary primets had good compatibility with DNA template of test strainsin RAPD assay. One of four was used to dentify by brids and their parents, three of fourwere used to identify fusants and their parents. The results showed that two of threehybrids were real heterozygotes,the two fusants were not stable.Therefore,comparing with the other ways,RAPD provided a rapid, simple, accurateidentified method of hybrids and fusants.
【中文關鍵詞】 本耳屬; RAPD; PCR; 雜合子; 融合子
【英文關鍵詞】 Auricularia; RAPD; PCR; Hybrid; Fusant
【基金】農業部“八五”生物技術攻關項目
【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1994年06期 【DOI】CNKI:SUN:ZSYJ.0.1994-06-000