林玉滿; 余萍; 劉艷如
福建師范大學實驗中心!福州350007; 福建師范大學高分子研究所!福州350007; 福建師范大學高分子研究所!福州350007
【中文摘要】 棘托竹蓀 (DictyophoraechinovolvataZang ,ZhengetHu)子實體干品經熱水抽提 ,乙醇沉淀 ,蛋白酶法和Sevag法去蛋白后得粗多糖DE。DE經乙醇分級沉淀得到三個級分DE1、DE2和DE3。DE2經DEAE SephadexA - 2 5柱進一步純化得到棘托竹蓀多糖純品DE2 - 2。DE2 - 2經高效液相色譜法測定為均一物質。紅外掃描表明 ,其具有典型的多糖吸收峰。紙層析和氣相色譜分析結果表明 ,DE2 - 2的單糖組成為 :D -葡萄糖、D -甘露糖、D -半乳糖和L -巖藻糖 ,其摩爾比為 8.68∶1.0 0∶1.85∶0 .74 ,分子量約為 84 0 0 0。經測定 ,DE2 - 2具有一定的抑瘤活性 ,其抑瘤率為38.93%。
【英文摘要】 The crude polysaccharide DE was extracted from dried fruitbodies of Dictyophora echinovolvata by hot water extraction, ethanol precipitation and deproteinization with protease method and Sevag method.Then three fractions DE1, DE2 and DE3 were obtained by fractional precipitation of DE with ethanol. While pure DE2-2 was obtained by further purification of DE2 with DEAE Sephadex A-25 and its homogeneity was verified by HPLC. And typical absorption peaks of polysaccharide were found with IR-scan. It was proved that DE2-2 was composed of D glucose, D mannose, D galactose and L fucose with a molar ratio of 8.68∶1.00∶1.85∶0.74 by paper and gas phase chromatography. The molecular weight of DE2-2 was estimated to be 84000 with an inhibition rate of 38.93% to Sarcoma-180 implanted in mice.
【中文關鍵詞】 多糖; 竹蓀; 純化; 抑瘤; 真菌
【英文關鍵詞】 Polysaccharide; Dictyophora echinovolvata; Purification; Sarcoma-180-inhibition; Fungus
【基金】福建省教委資助項目
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2001年01期 【DOI】CNKI:SUN:SYJB.0.2001-01-003