宋頻然; 常繼東
華東理工大學生物反應器工程國家重點實驗室; 華東理工大學生物反應器工程國家重點實驗室 上海200237 ; 上海200237
【中文摘要】 運用反饋抑制理論構建了耐靈芝胞外多糖 (EPS)反饋抑制的篩選模型。添加在篩選培養基中的其指示因子同源胞外多糖濃度為 2 .34g/L。將實驗室保藏的 37株靈芝菌株輸入該模型 ,即可檢出耐靈芝胞外多糖反饋抑制作用最強、產胞外多糖能力最強的菌株GL0 2 9。然后在基礎培養基的基礎上用正交試驗方法優化GL0 2 9深層發酵培養基。結果表明 ,組合碳源和組合氮源培養基最適合該菌株深層發酵生產靈芝胞外多糖。深層發酵培養基配方為 :蔗糖 10 g/L ,玉米粉15 g/L ,蛋白胨 2 g/L ,酵母膏 1g/L ,KCl 0 .5g/L ,KH2 PO4 ·7H2 O 0 .5 g/L ,pH自然。于 30℃、12 0r/min搖瓶培養 9d ,該菌株的胞外多糖產量高達 3.0 7g/L。
【英文摘要】 The theory of feedback inhibition was used to construct the screening model of Ganoderma lucidum extracellular polysaccharide (EPS) feedback inhibition resistant. And the concentration of its indicating factor, the homologous EPS added in the screening medium, was proved to be 2.34g/L. While GL029, the best G.lucidum EPS feedback inhibition resistant strain, namely the strain with the highest EPS productivity,was selected when 37 G. lucidum strains preserved in our laboratory were put into the screening model. Then the orthogonal test was used to optimize the submerged culture medium for GL029. The experimental results showed that the combined C sources and N sources were the optimum nutritional sources for the submerged culture of GL029 to produce EPS, and that 3.07g/L EPS could be produced when GL029 was cultured for 9d under the environment conditions of natural pH, 30℃ and with a rocking rate of 120r/min, by the medium of sucrose 10g/L, corn meal 15g/L, peptone 2g/L, yeast extract ...更多1g/L, KCl 0.5g/L, KH 2PO 4·7H 2O 0.5 g/L and water 1000mL. 還原
【中文關鍵詞】 靈芝; 靈芝胞外多糖; 反饋抑制; 高產菌株篩選; 培養基優化
【英文關鍵詞】 Ganoderma lucidum; Extracellular polysaccharide of G.lucidum; Feedback inhibition; Screening of strain with high productivity; Optimization of the medium
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2003年02期 【DOI】CNKI:SUN:SYJB.0.2003-02-002