黃春燕; 單洪濤; 劉傳富; 皇傳華; 萬魯長; 郭惠東; 楊化恩; 張福進
山東省農科院植保所; 山東省農科院植保所; 濟南市農科所; 濟南市農科所; 山東省農科院植保所; 山東省農科院植保所; 臨沂市河東區農業局; 威海市環翠區植保站 濟南250100 ; 濟南250100 ; 濟南250023 ; 濟南250023 ; 濟南250100 ; 濟南250100 ; 臨沂276000 ; 威海264000
【中文摘要】 研究了糙皮側耳液體菌種適宜的培養條件、培養周期與貯存期。結果表明,糙皮側耳液體菌種適宜的培養基配方為蔗糖2%,玉米粉4%,麥麩3%,酵母粉0.3%,KH2PO40.25%,Mg SO4·7H2O0.1%,ZnSO40.05%,pH6.17。搖瓶培養裝液量80mL/250mL,搖床轉速150r/min,接種量10%,培養周期6d,菌種于3d內用完為宜。
【英文摘要】 The suitable culture conditions, the culture period and the preservation time of the liquid spawn of P. ostreatus were studied. The experiment results showed that the optimum formula for P. ostreatus liquid spawn medium was as follows: sucrose 2%, corn meal 4%, wheat bran 3%, yeast powder 0.3%, KH_2PO_4 0.25%, MgSO_4·7H_2O 0.1%, ZnSO_4 0.05%, and the optimum pH was 6.17. While the optimum medium capacity, the rotation rate, the spawning rate and the culture period were 80mL/250mL, 150r/min,10% and 6d resp.. And it would be better to use the spawn within 3 days.
【中文關鍵詞】 糙皮側耳; 液體菌種; 培養條件; 培養周期; 貯存期
【英文關鍵詞】 Pleurotus ostreatus; Liquid spawn; Culture condition; Culture period; Preservation time
【基金】山東省濟南市科技局科技攻關項目"食用菌菌種引進選育及研究"[濟科合字(2001)第70-1號]的部分內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-009