邢增濤; 孫芳芳; 劉景圣
農業部食用菌產品質量監督檢驗測試中心(上海) 農業部食用菌遺傳育種重點開發實驗室 上海市農業遺傳育種重點實驗室 上海市農業科學院食用菌研究所; 南京農業大學資環學院; 吉林農業大學食品科技學院 上海201106 ; 南京210095 ; 長春130118
【中文摘要】 尖頂羊肚菌液體靜止培養的優化培養基為:蔗糖4%,胰蛋白胨0.4%,酵母粉0.15%,天門冬酰胺0.08%,MgSO4·7H2O0.5g/L,KH2PO41.45g/L,Na2HPO4·2H2O0.48g/L,VB125mg/L,CaCl20.013g/L,微量元素溶液1.0mL/L。培養基初始pH7.0,裝量50mL/250mL,接種量2%,培養溫度26℃。培養10d菌絲體生物量可達12.64g/L。
【英文摘要】 The experimental results showed that the optimized medium for static submerge-cultured (Morchella conica was) composed of sucrose 4%, tryptone 0.4%, yeast extract powder 0.15%, L-asparagine (0.08%, )MgSO_4·7H_2O 0.5g/L,KH_2PO_4 1.45g/L,Na_2HPO_4·2H_2O 0.48g/L,VB_1 25mg/L,CaCl_2 0.013g/L, and element solution 1.0mL/L. And the optimal initial pH, medium capacity, inoculation rate and culture temperature were 7.0, 50mL/250mL, 2% and 26℃ respectively. And the mycelial biomass of Morchellaconica can reach as high as 12.64g/L after inoculation 10 days.
【中文關鍵詞】 尖頂羊肚菌; 液體靜止培養; 生物量; 正交實驗
【英文關鍵詞】 Morchella conica; Static submerge-culture; Mycelial biomass; Orthogonal test
【基金】上海市農業科學院發展基金項目"羊肚菌雙歧桿菌混菌發酵技術的研究"的部分內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-008