周全; 唐興國; 姚志偉; 高軍偉
武漢生物工程學院生物工程系; 武漢生物工程學院生物工程系; 武漢生物工程學院生物工程系; 武漢生物工程學院生物工程系 湖北武漢430415; 湖北武漢430415; 湖北武漢430415; 湖北武漢430415
【中文摘要】 通過正交試驗篩選出適宜培養姬松茸液體菌種的合成培養基(N6Ⅰ20.0%,N6Ⅱ30.0%,蔗糖5.0%,酵母粉0.3%),以此制備的一級搖瓶種和二級搖瓶種,其菌絲體生物量分別為1.48±0.06g/100mL和2.64±0.05g/100mL。栽培試驗表明,液體菌種的發菌時間比固體菌種縮短13d。
【英文摘要】 A synthetic liquid medium consisting of 20.0% N6Ⅰ(mineral salt solution), 30.0% N6 Ⅱ(vitamin/amino acid supplement), 5.0% sucrose, 0.3% yeast powder was selected by orthogonal testing for culturing Agaricus blazei mycelium. Mycelial yields from shaken primary cultures (50 mL culture medium in 250 mL culture bottles) incubated at 25 ℃ for five days were 1.48±0.06 g/100 mL. Mycelial yields obtained following transfer of mycelium from primary cultures to secondary cultures (70 mL in 250 mL culture bottles) incubated at 25 ℃ for five days were 2.64±0.05 g/100 mL. Colonization of cultivation substrate using the liquid spawn from secondary cultures occurred 13 d earlier compared with normal stock spawn.
【中文關鍵詞】 姬松茸; N6培養基; 搖瓶培養; 液體菌種
【英文關鍵詞】 Agaricus blaze Murrill; N6 culture medium; Shaking bottle cultivation; Liquid spawn
【基金】武漢市市屬高等學校2006年度科學研究項目“巴西蘑菇標準化生產體系的建立”[武教高(2006)5號]的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2007年02期 【DOI】CNKI:SUN:SYJB.0.2007-02-019