沈霞; 余勝光
嘉應學院梅州師范分院
【中文摘要】 研究了稀土元素鑭(La)對灰樹花菌絲體富集有機硒的影響。結果表明,鑭對灰樹花菌絲富硒有促進作用,當玉米培養基中鑭添加濃度為100 mg/L時,菌絲產量和有機硒轉化率最高。借用正交試驗表設計組合試驗,結果表明,采用馬鈴薯培養基,培養時間8 d,鑭和硒的添加濃度分別為100 mg/L和50 mg/L,在此條件下,菌絲產量19.52 g/L,硒轉化率達16.48%。
【英文摘要】 The effect of lanthanum supplementation on selenium accumulation by Grifola frondosa mycelium grown in submerged culture was evaluated.When grown in a corn powder-based medium supplemented with different concentrations of lanthanum(20-300 mg/L),the maximum mycelial yield achieved was 18.58 mg/L,and 15.72% of the selenium initially present in the culture medium(50 mg/L) accumulated in the fungal mycelium.Combination tests involving fungal growth in four types of basal media(wheat bran,soybean,potato,corn powder) supplemented with different concentrations of selenium and lanthanum,and for different culture periods,revealed that the optimal conditions for selenium accumulation were eight days growth in a potato-based medium supplemented with 100 mg/L La and 50 mg/L Se.Under these conditions,the mycelial growth yield was 19.52 g/L,and 16.48% of the added Se accumulated in the fungal biomass.
【中文關鍵詞】 灰樹花; 液體培養; 鑭; 硒
【英文關鍵詞】 Grifola frondosa; submerged culture; lanthanum supplementation; selenium accumulation
【基金】梅州市科技計劃項目“灰樹花深層發酵培養基配方的優化研究”(編號:2006B30)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-020