孫巍; 夏春雨; 趙祥杰; 陳智毅; 鄒宇曉; 施英; 劉學銘
廣東省農業科學院蠶業與農產品加工研究所廣東省農產品加工公共實驗室
【中文摘要】 優化了以桑葉和麩皮為主要原料由毛云芝菌(Coriolus hirsutus)生產漆酶的培養條件,即培養料起始pH、裝料量、封口情況、接種量、發酵時間對漆酶活性的影響,并觀察了該菌在優化后培養條件下的代謝動力學情況。結果表明,自然pH,裝量為10 g/250 mL三角瓶、封口紗布4層,接種量為5 mL種子液/瓶,培養時間為10 d,酶活達到1.37×104U,優化后結果比初始設計提高了18%。
【英文摘要】 Substrate composition,initial pH,ratio of substrate to cultivation vessel volume,aeration,inoculum size and cultivation time were optimized for maximal laccase production by Coriolus hirsutus grown under solid-state conditions using mulberry leaves and wheat bran as the main components of the cultivation substrate.Highest laccase levels(1.37 × 104 U per) were recorded after ten days in 250 mL culture flasks containing 10 g substrate at pH 6,sealed with four layers of cheese cloth and inoculated with 5 mL of seed culture.
【中文關鍵詞】 Coriolus hirsutus; 漆酶; 培養條件
【英文關鍵詞】 Coriolus hirsutus; laccase; optimized cultivation conditions
【基金】教育部留學回國科研啟動基金(教外司留[2007]1108號);; 廣東省科技攻關項目(編號:2006B13001004)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2008年02期 【DOI】CNKI:SUN:SYJB.0.2008-02-017