劉開輝; 鄧百萬; 陳文強; 丁小維; 彭浩; 解修超
陜西理工學院生物科學與工程學院陜西省食藥用菌工程技術研究中心
【中文摘要】 對不同形態豬苓(Polyporus umbellata)菌核(豬屎苓、鐵蛋豬屎苓和雞屎苓)的rDNA(18S r DNA,ITS1-5.8S rDNA-ITS2)和β-tub1進行擴增、測序。序列分析表明:所得rDNA和β-tub1序列完全相同。以Neighbor-joining(N-J)方法構建ITS(ITS1-5.8S rDNA-ITS2)序列系統發育樹,供試菌株同GenBank中收錄豬苓(P.umbellata)類聚于同一分枝且序列完全相同,表明不同形態菌核的豬苓之間親緣關系較近。
【英文摘要】 Genetic relationships between three Polyporus umbellata strains,designated Zhushiling,Tiedanzhushiling and Jishiling on the basis of differences in sclerotium morphology,were analysed using rDNA(18S rDNA,ITS1-5.8S rDNA-ITS2) and β-tub1 fragment sequences.Although the test strains exhibited dissimilar sclerotial features,no differences were detected in their rDNA and β-tub1 sequences.Using the neighbor-joining method,a phylogenetic tree was constructed on the basis of the ITS data and other ITS sequences deposited in GenBank.This revealed a relatively high genetic similarity between the test strains,another P.umbellata strain and several Grifola umbellata strains.
【中文關鍵詞】 豬苓; 菌核; rDNA序列; β-tub1序列; 親源關系
【英文關鍵詞】 Polyporus umbellata; sclerotium; rDNA sequence; β-tub1 sequence; genetic relationship
【基金】陜西省教育廳項目(編號:04JS32,08JZ21,08JK244,08JK248);; 陜西理工學院人才引進項目(編號:SLGQDO712,SLGQDO713)的部分研究內容
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-003