雷萍; 黃志英; 林淼; 楊海華; 陳玉婷
上海市農業科學院農產品質量標準與檢測技術研究所; 上海市飼料行業協會; 上海儀表電子工業環境監測站
【中文摘要】 本實驗采用超高壓高效液相檢測法(UPLC)分離測定了多種蟲草產品中腺苷和蟲草素的含量。在最優化條件下,以ACQUITY UPLC C18為色譜柱,流動相為乙腈和水,等度洗脫,上述各組分在4 min內可基本實現基線分離。各組分濃度與峰面積在117.5μg/mL范圍內呈良好線性,檢測下限(S/N=3)分別為0.10μg/mL和0.12μg/mL。采用該方法成功測定了多種蟲草產品中腺苷和蟲草素的含量。
【英文摘要】 Adenosine and cordycepin levels in twelve Cordyceps products were successfully determined by Ultrahigh-Performance Liquid Chromatography(UPLC) using an ACQUITY C18 column,with water and acetonitrile as the mobile phase.Separation of the two components was achieved within four minutes,and the concentrations of each component showed a linear relationship with the peak area in the range 2～20 μg/mL.Detection limits(S/N=3) were 0.10 μg/mL and 0.12 μg/mL for adenosine and cordycepin,respectively.
【中文關鍵詞】 UPLC; 腺苷; 蟲草素
【英文關鍵詞】 Ultrahigh-Performance Liquid Chromatography; adenosine; cordycepin
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2009年03期 【DOI】CNKI:SUN:SYJB.0.2009-03-016