銀耳的超聲波介導轉化
Transformation of Tremella fuciformis by Using Ultrasonic Mediation
謝寶貴 饒永斌 鄭金貴
摘 要：以銀耳(Tremellafuciformis)芽孢為實驗材料,正交實驗研究超聲波處理時間、間歇時間及功率對轉化率的影響.實驗結果表明:超聲波處理時間與間歇時間對質粒DNA和芽孢轉化率都有顯著影響;實驗中質粒DNA轉化率最高達到754.7個轉化子/μg DNA,銀耳芽孢轉化率達到1.28‰;轉化子的潮霉素抗性可達到200μg/mL,GUS檢測陽性.
關鍵詞：銀耳;超聲波介導轉化;正交實驗
基金項目：福建省科技項目(No.2001Z010)基金資助.
作者簡介：謝寶貴:男,1962年生,博士,副教授.E-mail:＜xbg@pub2.fz.fj.cn＞.鄭金貴:通訊作者.Author for correspondence.E-mail:＜jinggui@fjau.edu.cn＞.
作者單位：謝寶貴（福建農林大學生命科學學院,福州,350002;福建農林大學菌物研究中心,福州,350002）
饒永斌（福建農林大學生命科學學院,福州,350002;福建農林大學菌物研究中心,福州,350002）
鄭金貴（福建農林大學農產品品質研究所,福州,350002）
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農業生物技術學報
JOURNAL OF AGRICULTURAL BIOTECHNOLOGY
2005 Vol.13 No.1 P.42-45
