猴頭菌多糖罐批發酵種子培養及發酵條件優化
Cultivation of Seeds and Optimization of Conditions for Batch Fermentation of Hericium erinaceus Polysaccharide
【作者】 張筱梅; 朱維紅; 苗曉燕;
【Author】 ZHANG Xiao-mei,ZHU Wei-hong,MIAO Xiao-yan (Department of Biochemistry,Baoding University,Hebei Baoding 071000)
【機構】 河北保定學院生化系;
【摘要】 試驗確定了猴頭多糖罐批發酵的種子培養基配方、種子質量標準、發酵控制條件及發酵終點。猴頭罐批培養要求種子活力旺盛,菌球色白,懸浮性強,密度3.0×103個.mL-1~4.0×103個.mL-1,直徑0.5 mm~1.5 mm。5批發酵表明,猴頭多糖培養以26℃,pH5.0,攪拌150 r.min-1~200 r.min-1,罐壓0.04 PMa~0.06 PMa,通風0.6 m3.h-1~0.8 m3.h-1培養144 h~168 h較適宜,菌絲多糖達115 mg.g-1~130 mg.g-1。比較發現,猴頭罐批發酵較栽培子實體多糖高2倍。
【Abstract】 A recipe of substrate for Hericium polysaccharide seeds in fermenters,the quality index of seeds,the ferment conditions and termination conditions had found.Hericium in fermenters required thriving seeds,white fungus,high degree of suspension,with density of 3.0×103~4.0×103 per mL and diameter of 0.5 mm~1.5 mm.Five batches of ferment experiments had shown that when the cultivation of Hericium polysaccharide under 26℃,pH5.0,stirred at 150 r·min-1~200 r·min-1,with pressure of 0.04 MPa~0.06 MPa and...
【關鍵詞】 猴頭菌; 種子指標; 發酵罐; 多糖; 培養條件;
【Key words】 Hericium erinaceus; Seed index; Fermenter; Polysaccharide; Condition;
【基金】 河北省教育廳計劃指導研究項目(Z2008401)
【分類號】S646.9