許襄中; 夏道平
武漢市蔬菜科研所!武漢430065; 武漢市蔬菜科研所!武漢430065
【中文摘要】 本研究對無孢平菇菌株P22原生質體進行紫外線誘變處理,經拮抗試驗,孢子印檢測,掃描電鏡和出菇試驗,篩選出一株中低溫型、高產、菇形好的無孢平菇突變菌株4-10。品比結果顯示,4-10新菌株兩年平均生物效率達112％,較出發菌株P22增產19.8％,較對照菌株P51增產22.7％。研究結果表明,原生質體紫外線誘變是一種快速簡便且有效的育種方法。
【英文摘要】 The sporeless, mid-low temperature type, high yield mutagenic strain of P. ostreatus 4-10 with fine fruitbody morphology was selected from sporeless P. ostreatus P22 protoplast through irradiation of UV ray, antagonistic test, spore print identification, electron microscope scanning and fruiting experiment. The average biological efficiency of the new strain 4-10 in two years was up to 112%, increased by a factor of 19.8 % over the parent strain P22 and 22.7 % over the control strain P51. The results showed that UV radiation of P. ostreatus protoplast was a rapid, simple and effective method for sporeless strain breeding.
【中文關鍵詞】 誘變育種; 無孢平菇; 原生質體再生; 紫外線
【英文關鍵詞】 Mutation brceding; Sporeless Pleurotus ostreatus; Protoplast regeneration
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1997年02期 【DOI】CNKI:SUN:SYJB.0.1997-02-003