王淑珍; 白晨; 范俊; 高雁; 揚家峰; 揚英杰
上海師范大學生命與環境科學學院; 復旦大學生命科學學院; 上海師范大學生命與環境科學學院; 上海師范大學生命與環境科學學院; 上海師范大學生命與環境科學學院; 上海師范大學生命與環境科學學院 上海200234 ; 上海200433 ; 上海200234 ; 上海200234 ; 上海200234 ; 上海200234
【中文摘要】 以獲得弱木栓質化靈芝菌株為目的 ,利用液體培養 4d的靈芝菌絲體和培養 3d的糙皮側耳菌絲體為材料 ,在PEG Ca2 +系統中誘導原生質體融合 ,獲融合子 19株 ,融合率為 1.9×10 -5。根據其生物學特性 ,選取F3、F8兩融合子及兩親本菌株的基因進行RAPD分析。結果表明 ,兩親本的核物質在融合子中發生了重組 ,并影響了酶蛋白的表達 ,而融合子在DNA水平上更接近靈芝
【英文摘要】 In order to obtain less corkification strain of G.lucidum, the mycelia of G.lucidum and P.ostreatus were put under submerged culture for 4d and 3d respectively to prepare the protoplast. The fusion experiment between G. lucidum and P. ostreatus protoplasts was carried out under PEG Ca 2+ system, and nineteen fusants were selected.The fusion frequency was as high as 1.9×10 -5 . According to their biological characters, RAPD analysis for genomes of fusants F3?F8 selected randomly and two parents was conducted. The results showed that the nuclear substance of two parents was recombined in the fusant and the expression of the enzyme protein was affected. The fusants were proved to be more similar to G.lucidum at DNA level.
【中文關鍵詞】 靈芝; 糙皮側耳; 原生質體; 融合; 融合子; RAPD分析; DNA譜帶
【英文關鍵詞】 Ganoderma lucidum; Pleurotus ostreatus; Protolast; Fusion; Fusant; RAPD analysis; DNA band.
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2003年01期 【DOI】CNKI:SUN:SYJB.0.2003-01-000