林玉滿
福建師范大學實驗中心!福州350007
【中文摘要】 人工栽培的竹蓀（Dictyophora indusiata）子實體干品經熱水提取,乙醇沉淀,蛋白酶法和Sevag法相結合去蛋白,DEAE-Sephadex A-25和Sephadex G-200柱純化,得到竹蓀多糖,命名為DI.用聚丙烯酰胺凝膠電泳和葡聚糖凝膠柱層析,分析表明:該多糖為均一物質.竹蓀多糖的分子量為144000.經紫外掃描,無核酸和蛋白質的特征吸收峰,紅外掃描表明有典型的多糖吸收峰.該多糖經紙層析和氣相色譜分析表明:其單糖組成為L-巖藻糖、D-木糖、D-甘露糖、D-葡萄糖,摩爾比為0.16∶0.24∶1.00∶1.08.
【英文摘要】 A polysaccharide DI was extracted from dried fruitbodies of Dictyophora indusiata by hot water. In the extract, it was precipitated by ethanol, deproteinized by Protease method and Sevag method and further purified on DEAE-Sephadex A-25 and Sephadex G-200, its homogeneity was verified by glucan gel filtration chromatography and polyacrylamide gel electrophoresis. No characteristic absorption of nucleic acid and protein was showed in polysaccharide UV spectrum, but type absorption of polysaccharide was showed in its IR spectrum. The components of DI, identified by paper chromatography and gas chromatography, were composed of L-fucose, D-xylose, D-mannose and D-glucose in the molar ratio of 0.16:0.24:1.00:1.08. And its molecular weight was estimated to be 144000.
【中文關鍵詞】 竹蓀; 多糖; 分離; 純化; 真菌
【英文關鍵詞】 Dictyophora indusiata’; Polysaccharide; Isolation; Purification; Fungus
【基金】福建省自然科學基金資助項目
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1995年01期 【DOI】CNKI:SUN:SYJB.0.1995-01-003