陳明杰; 趙紹惠; 張樹庭
上海市農業科學院食用菌所!上海201106; 香港中文大學生物系!沙田香港; 香港中文大學生物系!沙田香港
【中文摘要】 利用等高鎖狀同源電場凝膠電泳（CHEF）,獲得草菇的電泳核型.綜合兩組脈沖電泳的電泳條件、溴化乙錠染色以及DNA雜交分析結果,草菇染色體DNA至少可分為9個譜帶.這一結果和以前用光學顯微鏡觀察草菇的染色體數目的結果相一致.以混濁釀酒酵母和粟酒裂殖酵母等的相對遷移率為基準,草菇染色體長度估計在1.32～5.4Mb之間,染色體組總長約25Mb.
【英文摘要】 An electrophoretic karyotype of Volvariella volvacea was obtained using Contour-Clamped Homogeneous Electric Field (CHEF) gel electrophoresis. Based on the conditions of 2 sets of pulsed field gel electrophoresis, the results of ethidium bromide staining and DNA hybridization analysis with two moderately repetitive probes, it was concluded that at least 9 chromosomal DNAs were found in V. volvacea. This result was consistent with previous studies using light microscopy. The chromosome size was estimated as 1.32 Mb to 5.4 Mb based on gel migration relative to the chromosomes of Saccharomyces cerevisiae, Schizosaccharomyces pombe and Hansenula wingei strains. The total genome size of V. volvacea was estimated to be about 25 Mb.
【中文關鍵詞】 草菇; 染色體核型; 脈沖電泳
【英文關鍵詞】 Volvariella volvacea; Karyotype; Pulsed field gel electrophoresis
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1995年01期 【DOI】CNKI:SUN:SYJB.0.1995-01-000