詹才新; 朱蘭寶; 楊新美
華中農業大學!武漢430070; 華中農業大學!武漢430070; 華中農業大學!武漢430070
【中文摘要】 本文以金針菇（Flammulina velutipes）不同親本菌株及其雜交種為材料,用20個隨機引物進行了RAPD分析,結果表明,兩個隨機引物擴增出特異的DNA片段,經三次重復,均獲得相同結果,且能明顯地區別鑒定出真正的雜交種.因此,在雜交育種中,RAPD分析是一種簡便、快速、有效地從雜交后代中區別鑒定出真正雜交種的方法.
【英文摘要】 The random amplified polymophic DNA (RAPD) analysis was made in this study with 20 random primers in various parent strains and hybrids of Flammulina velanpes. The result indicated that two of them amplified the special polymorphic DNA fragment. The same results were obtained in all three replications of the experiment and rhe true hybrids were distinguished obviously from the progemy. Therefore, RAPD analysis was an effective method for rapid identification of the true hybrids.
【中文關鍵詞】 金針菇; RAPD分析; 雜交種
【英文關鍵詞】 Flammulina velutipes; RAPD analysis; Hybrid
【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,1995年01期 【DOI】CNKI:SUN:SYJB.0.1995-01-001